Mean corpuscular volume is one of the important indices in classifying the anemia
and determining the macrocytosis. In general, though the electronically determined
hematologic values are more accurate than that of conventional methods, the MCV
measured by electronical method is influenced by many diseases or states such as the
states of increased serum osmolality (liver disease, alcoholism, high titer coed
agglutinins, hyperglycemia, etc). Hence more precise evaluation in MCV is essential in
these condition.
Since hyperglycemic state increases the osmolality of the serum and intra-red blood
cell, the hyperglycemic red blood cell will expand and increase its volume when dilited
acutly, and this occurs during the measurement of hematologic values by electronic
counter.
Authors haute studied the relationship between blood glucose level and MCV
measured by conventional and electronical method in hyperglycemic patients who
admitted to Hanyang University Hospital, Seoul, Korea, from Jan. '83 to Dec. '83. And
also, experimentally, authors studied the changes of MCV in various blood glucose levels
by adding the glucose in blood obtained from healthy volunteers in fasting states.
The result were as follows ;
1. Mean MCV was significantly higher in hypergycemic patient than that of normal
control, both in male and female (p<0.05).
2. Hematocrit and MCV were significantly increased as blood glucose increased
(p<0.05, respectively), but no RBC count was changed significantly (p>0.05).
By least-squares method, the regression equation of the hematocrit and MCV were as
follows ;
HCT: Y=1.25X 37.7
MCV: Y=3.25X 89.0
3. When the temperature was increased from 4℃ to 37℃ hematotcrit and MCV were
increased when electronically measured (p<0.05, respectively), but no RBC count was
changed significantly (p>0.05).
4. The values of RBC count, hematocrit and MCV were no significantly differences
before and after adding the glucose when the red blood cells were suspended in the
diluent prior to measurement for more than five minutes.
5. When the hematocrit and MCV were measured sequentially (15, 30, 60min.) after
adding glucose, the hematocrit and MCV were increased significantly (p<0.05, for each
sequences), but no RBC count was changed significantly (P>0.05).
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